Polydatin induces bone marrow stromal cells migration by activation of ERK1/2 - 08/08/16

Doi : 10.1016/j.biopha.2016.04.059

ZhenQiu Chen ^a,^b,^{^{1
These authors contributed equally to this study.}}, QiuShi Wei ^a,^b,^{^{1
These authors contributed equally to this study.}}, GuoJu Hong ^b, Da Chen ^b, Jiang Liang ^a, Wei He ^a,^b,^⁎ , Mei Hui Chen ^b,^c,^⁎
^a Department of Orthopaedics, The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Baiyun District, Guangzhou, Guangdong 510405, China
^b Key Laboratory of Orthopaedics & Traumatology, Guangzhou University of Chinese Medicine, Guangzhou 510405, China
^c Guangdong Provincial Acadamy of Chinese Medical Sciences, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou 510120, China

^⁎Corresponding authors at: Key Laboratory of Orthopaedics & Traumatology, Guangzhou University of Chinese Medicine, Guangzhou 510405, China.Key Laboratory of Orthopaedics & TraumatologyGuangzhou University of Chinese MedicineGuangzhou510405China

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Abstract

Bone marrow stromal cells (BMSCs) have proven to be useful for the treatment of numerous human diseases. However, the reparative ability of BMSCs is limited by their poor migration. Polydatin, widely used in traditional Chinese remedies, has proven to exert protective effects to BMSCs. However, little is known about its role in BMSCs migration. In this study, we studied the effects of polydatin on rat BMSCs migration using the scratch wound healing and transwell migration assays. Our results showed polydatin could promote BMSCs migration. Further experiments showed activation of ERK 1/2, but not JNK, was required for polydatin-induced BMSCs migration, suggesting that polydatin may promote BMSCs migration via the ERK 1/2 signaling pathways. Taken together, our results indicate that polydatin might be beneficial for stem cell replacement therapy by improving BMSCs migration.

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Keywords : Polydatin, BMSCs, Migration, ERK 1/2

Plan

Introduction

Meterials and methods

Materials

Cell culture and treatment

MTT assay

Scratch wound healing assay

Transwell migration assay

Western blot

Statistical analyses

Results

Characterization of BMSCs

Polydatin promotes BMSCs migration

Polydatin increases ERK 1/2 phosphorylation but not JNK in BMSCs

Polydatin promotes BMSCs migration via activation of ERK 1/2 but not JNK

Discussion

Conclusion

Conflict of interest

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Vol 82

P. 49-53 - août 2016 Retour au numéro

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Polydatin induces bone marrow stromal cells migration by activation of ERK1/2 - 08/08/16

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