Host immune responses to antigens derived from a predominant strain of Mycobacterium tuberculosis - 10/06/16
, Sang-Nae Cho a, ⁎ Summary |
Objectives |
Beijing strain of Mycobacterium tuberculosis (M. tb) is characterized by a high incidence of transmission, relapse, and drug resistance. This study aimed to determine host immune responses to antigens derived from the Beijing/K strain which has been highly prevalent in tuberculosis (TB) outbreaks in South Korea.
Methods |
We recruited 52 TB patients and 96 healthy subjects comprising 31 individuals with latent TB infection (LTBI) and 65 TB-naïve controls based on QuantiFERON-TB Gold In-Tube (QFT-IT) tests. Blood samples were obtained for diluted whole-blood assays, multiplex bead arrays, ELISpot assays, and HLA typing. Molecular genotyping of M. tb was performed using clinical isolates.
Results |
Active TB and LTBI groups were differentiated by TNF-α concentrations induced by the Beijing/K strain-derived antigens, MTBK_24790 and MTBK_24800 (P < 0.001). MTBK_24800-induced IFN-γ and CXCL10 concentrations discriminated the TB-infected groups from TB-naïve controls (P < 0.001). IFN-γ-producing T cells were generated in 87.2% of TB patients in response to MTBK_24800 peptide antigens. The major immunogenic epitope was at C-terminal of the antigen, and predominantly recognized by HLA-DR4 and -DQ4.
Conclusions |
Measurement of IFN-γ, CXCL10, and TNF-α concentrations induced by MTBK_24790 and MTBK_24800 may contribute to improved diagnosis of TB and vaccine development in regions where the Beijing/K strain is endemic.
Le texte complet de cet article est disponible en PDF.Highlights |
• | Beijing/K strains of M. tb are highly transmissible and predominant in South Korea. |
• | MTBK_24790 and MTBK_24800 were identified from Beijing/K strains of M. tb. |
• | Active TB and LTBI were differentiated by the Beijing/K antigen-induced TNF-α. |
• | M. tb infection was detected by the MTBK_24800-induced IFN-γ and CXCL10. |
• | Beijing/K antigen-specific IFN-γ, CXCL10, and TNF-α may help the IGRA performance. |
Keywords : Beijing/K strain, CXCL10, IFN-γ, Latent TB infection, Mycobacterium tuberculosis, TB, TNF-α
Plan
Vol 73 - N° 1
P. 54-62 - juillet 2016 Retour au numéro
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