Thermostability of IFN-? and IP-10 release assays for latent infection with Mycobacterium tuberculosis: A TBnet study - 09/05/16

Doi : 10.1016/j.tube.2015.04.013

Thomas Blauenfeldt ^a, Dirk Wagner ^b, Martine Aabye ^c, Jan Heyckendorf ^d, Berit Lange ^b, Christoph Lange ^d,^e,^f,^g, Martin Ernst ^d, Pernille Ravn ^h, Raquel Duarte ^i,^j,^k,^l, Clara Morais ^m, Matthias Hoffmann ⁿ, Otto D. Schoch ^o, Jose Dominguez ^p, Irene Latorre ^p, Morten Ruhwald ^a,^⁎
^a Department of Infectious Disease Immunology, Statens Serum Institut, Copenhagen, Denmark
^b Department of Infectious Diseases and Center for Chronic Immunodeficiency, Medical Center, University of Freiburg, Freiburg, Germany
^c Department of Double Diagnosis, Mental Health Centre Sct. Hans, Roskilde, Denmark
^d Division of Clinical Infectious Diseases, Research Center Borstel, Germany
^e German Center for Infection Research Tuberculosis Unit International Health/Infectious Diseases, University of Lübeck, Germany
^f Department of Internal Medicine, University of Namibia School of Medicine, Windhoek, Namibia
^g Department of Medicine, Karolinska Institute, Stockholm, Sweden
^h Department of Pulmonary and Infectious Diseases, Nordsjaelland Hospital, Hillerød, Denmark
ⁱ Tuberculosis Outpatient Centre Vila Nova de Gaia, Portugal
^j Hospital Centre of Vila Nova de Gaia/Espinho, Portugal
^k Medical School, Porto University, Portugal
^l Institute of Public Health, Porto University, Portugal
^m Pulmonology Diagnostic Center Porto and Vila Nova de Gaia, Portugal
ⁿ Division of Infectious Diseases and Hospital Epidemiology, Cantonal Hospital St.Gallen, Switzerland
^o Division of Pneumology, Department of Internal Medicine, Cantonal Hospital St.Gallen, Switzerland
^p Servei de Microbiologia, Institut d’Investigació en Ciències de la Salut Germans Trias i Pujol, Universitat Autònoma de Barcelona, Ciber Enfermedades Respiratorias, Instituto de Salud Carlos III, Badalona, Spain

^∗Corresponding author. Statens Serum Institut, Department of Infectious Disease Immunology, Building 81 – 3rd floor, Artillerivej 5, 2300 S., Copenhagen, Denmark. Tel.: +45 32683940.

Summary

Introduction

Interferon-γ (IFN-γ) inducible protein 10kD (IP-10) and IFN-γ release assays (IGRAs) are immunodiagnostic tests aiming to identify the presence of specific cellular immune responses, interpreted as markers for latent infection with Mycobacterium tuberculosis. Incubation at higher temperatures could affect IFN-γ and IP-10 responsiveness in order to improve the performance of IP-10 release assays and IGRAs.

Aim

The aim of this study was to assess the robustness of whole blood based IP-10 release assay and IGRAs and the effect of hyper-thermic incubation (39 °C) on the diagnostic accuracy of IP-10 release assay and IGRAs.

Results

We included 65 patients with confirmed pulmonary tuberculosis and 160 healthy controls from 6 European centres collaborating in the TBnet. In patients, IP-10 responses increased 1.07 (IQR 0.90–1.36) fold and IFN-γ responses decreased 0.88 (IQR 0.57–1.02) fold, with 39 °C compared to 37 °C incubation temperature. At 37 °C IGRA sensitivity was 85% and IP-10 sensitivity was 82%, whereas specificity was 97% for both tests (p > 0.8). These minor changes observed as a result of hyper-thermic incubation were not sufficient to impact IGRA and IP-10 release assay test performance.

Conclusion

The performance of IGRA and IP-10 release assays is robust despite variations in the incubation temperature between 37 °C and 39 °C.

Le texte complet de cet article est disponible en PDF.

Keywords : IGRA, IP-10 release assay, Hyperthermia, Mycobacterium. tuberculosis

Plan

Biomarker responses: 39 °C vs. 37 °C

ROC curve analysis

Test results, concordance and changes in positivity rates: 39 °C vs. 37 °C

Discussion

Conclusion

Conflicts of interest

Export

Vol 98

P. 7-12 - mai 2016 Retour au numéro

Article précédent

Unraveling methylation changes of host macrophages in Mycobacterium tuberculosis infection
Lin Zheng, Eric T.Y. Leung, H.K. Wong, Grace Lui, Nelson Lee, Ka-Fai To, K.W. Choy, Raphael C.Y. Chan, Margaret Ip

| Article suivant

Impaired NK cells' activity and increased numbers of CD4 + CD25+ regulatory T cells in multidrug-resistant Mycobacterium tuberculosis patients
Renhua Fan, Yangen Xiang, Li Yang, Yanke Liu, Pingsheng Chen, Lei Wang, Wenjun Feng, Ke Yin, Manjiao Fu, Yixin Xu, Jialin Wu

Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.

Déjà abonné à cette revue ?

connectez-vous ou créez un compte

Thermostability of IFN-? and IP-10 release assays for latent infection with Mycobacterium tuberculosis: A TBnet study - 09/05/16

Summary

Introduction

Aim

Results

Conclusion

Plan

Export citations

Fichier

Contenu

Accès rapides

Mon compte

Aide & support

Plateformes Elsevier Masson

Déclaration CNIL