Surface functionalization of oxidized multi-walled carbon nanotubes: Candida rugosa lipase immobilization - 07/04/16
, Dejan I. Bezbradica b , Jelena R. Rogan b , Petar S. Uskoković b , Dušan Ž. Mijin b , Aleksandar D. Marinković b Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 8 |
| Iconographies | 8 |
| Vidéos | 0 |
| Autres | 0 |
Abstract |
This work examines two approaches for immobilization of lipase from Candida rugosa on oxidized multi-walled carbon nanotubes (o-MWCNTs). One method included the presence of activating agents to promote covalent bonding and the other the adsorption on o-MWCNTs to elucidate if non-specific bonding on the o-MWCNTs surface exists. The influence of the immobilization time and initial enzyme concentration on protein loading and the expressed lypolitic activity of the immobilized preparation were investigated. The results showed that the enzyme adsorbs on o-MWCNTs in a maximal amount of 37 μg mg−1 CNTs, while the attached amount was more than 2-times higher under covalent promoting conditions (80 μg mg−1 CNTs). Furthermore, similar trends were observed for the lypolitic activity, whereby preparations obtained under covalent promoting conditions had almost 3-times higher activity (560 IU g−1 of immobilized enzyme). In addition, immobilization of the enzyme was confirmed by Fourier transformation infrared spectroscopy and thermogravimetric analysis.
Le texte complet de cet article est disponible en PDF.Graphical abstract |
Keywords : Candida rugosa lipase, Enzyme immobilization, Carbon nanotubes, o-MWCNT, Carbodiimide chemistry
Plan
Vol 19 - N° 3
P. 363-370 - mars 2016 Retour au numéro
Article précédent
- Electrochemical synthesis of new organic compounds based on the oxidation of 1,4-dihydroxybenzene derivatives in the presence of primary and secondary amines
- Davood Nematollahi, Hooman Hesari, Hamid Salehzadeh, Mahdi Hesari, Shima Momeni
- Manganese(III) porphyrin supported onto multi-walled carbon nanotubes for heterogeneous oxidation of synthetic textile dyes and 2,6-dimethylphenol by tert-butyl hydroperoxide
- Saeed Rayati, Zahra Sheybanifard
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?