Altered interferon-? response in patients with Q-fever fatigue syndrome - 09/03/16
, Ruud P.H. Raijmakers a, b , Chantal P. Bleeker-Rovers a, b , Jos W.M. van der Meer b , Mihai G. Netea b , Teske Schoffelen a, b , Marcel van Deuren b Summary |
Objectives |
Whether immunological mechanisms underlie Q-fever fatigue syndrome (QFS) remains unclear. For acute Q-fever, the antigen-specific interferon-γ (IFNγ) response may be a useful tool for diagnosis, and the IFNγ/interleukin(IL)-2 production ratio may be a marker for chronic Q-fever and treatment monitoring. Here we explored the specific IFNγ production and IFNγ/IL-2 ratio in QFS patients.
Methods |
IFNγ and IL-2 production were tested in ex-vivo stimulated whole blood of QFS patients (n = 20), and compared to those previously determined in seropositive controls (n = 135), and chronic Q-fever patients (n = 28). Also, the correlation between patient characteristics and IFNγ, IL-2, and IFNγ/IL-2 ratio was determined.
Results |
QFS patients were younger (p < 0.001), but gender distribution was similar to seropositive controls and chronic Q-fever patients. Coxiella burnetii Nine Mile stimulation revealed a higher IFNγ production in QFS (median 319.5 pg/ml) than in seropositive controls (120 pg/ml, p < 0.01), but comparable to chronic Q-fever (2846 pg/ml). The IFNγ/IL-2 ratio was similar to that in seropositive controls, but lower than in chronic Q-fever patients (p < 0.01). Symptom duration was positively correlated with IL-2 production, and negatively correlated with the IFNγ/IL-2 ratio.
Conclusions |
These results point to an altered cell-mediated immunity in QFS, and suggest a different immune response than in chronic Q-fever.
Le texte complet de cet article est disponible en PDF.Highlights |
• | We explored the specific IFNγ production, and the IFNγ/IL-2 ratio in QFS patients. |
• | QFS patients have a significant higher IFNγ production than seropositive controls. |
• | The IFNγ/IL-2 ratio is significantly lower in QFS than in chronic Q-fever patients. |
• | These results point to an altered cell-mediated immunity in QFS. |
Keywords : Cell-mediated immunity, Coxiella burnetii, Cytokines, Diagnosis, Interferon-gamma, IFNγ, Q-fever, Q-fever fatigue syndrome, QFS
Plan
Vol 72 - N° 4
P. 478-485 - avril 2016 Retour au numéro
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