Extracellular vesicle–derived protein from Bifidobacterium longum alleviates food allergy through mast cell suppression - 05/02/16
Abstract |
Background |
The incidence of food allergies has increased dramatically during the last decade. Recently, probiotics have been studied for the prevention and treatment of allergic disease.
Objective |
We examined whether Bifidobacterium longum KACC 91563 and Enterococcus faecalis KACC 91532 have the capacity to suppress food allergies.
Methods |
B longum KACC 91563 and E faecalis KACC 91532 were administered to BALB/c wild-type mice, in which food allergy was induced by using ovalbumin and alum. Food allergy symptoms and various immune responses were assessed.
Results |
B longum KACC 91563, but not E faecalis KACC 91532, alleviated food allergy symptoms. Extracellular vesicles of B longum KACC 91563 bound specifically to mast cells and induced apoptosis without affecting T-cell immune responses. Furthermore, injection of family 5 extracellular solute-binding protein, a main component of extracellular vesicles, into mice markedly reduced the occurrence of diarrhea in a mouse food allergy model.
Conclusion |
B longum KACC 91563 induces apoptosis of mast cells specifically and alleviates food allergy symptoms. Accordingly, B longum KACC 91563 and family 5 extracellular solute-binding protein exhibit potential as therapeutic approaches for food allergies.
Le texte complet de cet article est disponible en PDF.Key words : Food allergy, Bifidobacterium longum, probiotics, extracellular vesicle, mast cells, family 5 extracellular solute-binding protein, ovalbumin
Abbreviations used : ASBP, BMMC, cfu, DC, DC-SIGN, ESBP, EV, FISH, Foxp3, GST, LP, MCPT-1, MHCII, MLN, OVA, TCR, TEM, Treg, TUNEL
Plan
Supported by the Institute for Basic Science (IBS; IBS-R005-S1-2015-a00 and IBS-R005-D1-2015-a00), the National Institute of Animal Science research project of Rural Development Administration of Korea (PJ00932901), and the National Research Foundation of Korea (NRF; 2013-056085). |
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Disclosure of potential conflict of interest: The authors declare that they have no relevant conflicts of interest. |
Vol 137 - N° 2
P. 507 - février 2016 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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