IFN-? and IgA against non-methylated heparin-binding hemagglutinin as markers of protective immunity and latent tuberculosis: Results of a longitudinal study from an endemic setting - 29/01/16
, Mengistu Legesse a, Adane Mihret c, d, Tom H.M. Ottenhoff e, Kees L. Franken e, Gunnar Bjune b, Fekadu Abebe bSummary |
Background |
Heparin-binding hemagglutinin (HBHA) is a surface protein involved in epithelial attachment and extrapulmonary dissemination of Mycobacterium tuberculosis. HBHA is attracting increasing attention for its vaccine and diagnostic potential. In a longitudinal study, we investigated non-methylated, recombinant HBHA-specific cytokine and antibody profiles in cohorts of TB patients, their contacts and community controls in an endemic setting.
Methods |
Whole blood assay was done at baseline, 6 and 12 months in patients and contacts, and at entry in controls. ELISA was used to measure IFN-γ, TNF-α and IL-10 (from supernatants), and IgG, IgM and IgA (from sera).
Results |
Fifty-three percent of controls and 72.1% of contacts were QFT-GIT positive. Baseline IFN-γ was significantly higher in community controls and contacts compared to untreated TB patients (p < 0.0001). Controls had significantly higher IgA and lower IgM compared to both untreated TB patients and contacts (p < 0.0001). IL-10 was significantly higher in untreated TB patients compared to contacts and controls (p < 0.0001). In treated TB patients, IFN-γ significantly increased (p < 0.0001) whereas IL-10 significantly decreased (p < 0.001).
Conclusion |
This study reports for the first time that anti-HBHA IgA could have the potential as a biomarker of protective immunity. In addition, non-methylated, recombinant HBHA-induced IFN-γ could be used as a biomarker of protective immunity and latent TB.
Le texte complet de cet article est disponible en PDF.Highlights |
• | HBHA-specific cytokines and antibodies were compared in TB patients, their contacts and community controls. |
• | Untreated TB patients had significantly lower IFN-γ and higher IL-10 compared to both contacts and community controls. |
• | Community controls had significantly higher anti-HBHA IgA compared to both untreated TB patients and contacts. |
• | Non-methylated HBHA-specific IFN-γ could be used as a biomarker of latent TB and protective immunity. |
• | Anti-HBHA IgA could have the potential as a novel biomarker of protective immunity. |
Keywords : Tuberculosis, Patients, Contacts, Community controls, HBHA, IFN-γ, IL-10, IgG, IgM, IgA
Plan
Vol 72 - N° 2
P. 189-200 - février 2016 Retour au numéro
Article précédent
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