Population tailored modification of tuberculosis specific interferon-gamma release assay - 29/01/16
Summary |
Objectives |
Blood-based Interferon-Gamma Release Assays (IGRA) identify Mycobacterium tuberculosis (MTB) sensitisation with increased specificity, but sensitivity remains impaired in human immunodeficiency virus (HIV) infected persons. The QuantiFERON-TB Gold In-Tube test contains peptide 38–55 of Rv2654c, based on data indicating differential recognition between tuberculosis patients and BCG vaccinated controls in Europe. We aimed to fine map the T cell response to Rv2654c with the view of improving sensitivity.
Methods |
Interferon-gamma ELISpot assay was used in HIV uninfected persons with latent and active tuberculosis to map peptide epitopes of Rv2654c. A modified IGRA was tested in two further groups of 55 HIV uninfected and 44 HIV infected persons, recruited in South Africa.
Results |
The most prominently recognised peptide was between amino acids 51–65. Using p51-65 to boost the QuantiFERON-TB Gold In-Tube assay, the quantitative performance of the modified IGRA increased from 1.83 IU/ml (IQR 0.30–7.35) to 2.83 (IQR 0.28–12.2; p = 0.002) in the HIV uninfected group. In the HIV infected cohort the percentage of positive responders increased from 57% to 64% but only after 3 months of ART (p = ns).
Conclusions |
Our data shows the potential to population tailor detection of MTB sensitization using specific synthetic peptides and interferon-gamma release in vitro.
Le texte complet de cet article est disponible en PDF.Highlights |
• | Refined epitope mapping of Rv2654c was performed in a South African Xhosa population. |
• | The most frequently recognized peptide is p51-65 (VRAVAESHGVAAVLF). |
• | Using p51-65 in the QFT assay resulted in a significant boosting effect. |
• | Peptide p51-65 can improve the population tailored detection of MTB sensitization. |
Keywords : Tuberculosis, Interferon-Gamma Release Assays (IGRA), QuantiFERON assay, Peptide epitope, Rv2654c, HIV
Plan
Vol 72 - N° 2
P. 179-188 - février 2016 Retour au numéro
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