The effect of HIV coinfection, HAART and TB treatment on cytokine/chemokine responses to Mycobacterium tuberculosis (Mtb) antigens in active TB patients and latently Mtb infected individuals - 17/01/16
, Wilco de Jager c , Gebremedhin Gebremichael a , Yodit Alemayehu a , Leonie Ran b , Justin Fransen b , Dawit Wolday d , Tsehaynesh Messele a , Belete Tegbaru a , Tom H.M. Ottenhoff e , Debbie van Baarle b, 1 Summary |
Identification of Mtb specific induced cytokine/chemokine host biomarkers could assist in developing novel diagnostic, prognostic and therapeutic tools for TB.
Levels of IFN-γ, IL-2, IL-17, IL-10, IP-10 and MIP-1α were measured in supernatants of whole blood stimulated with Mtb specific fusion protein ESAT-6/CFP-10 using xMAP technology. The study groups were HIV positive TB patients (HIV+TB+), HIV negative TB patients (HIV−TB+), HIV positive tuberculin skin test positive (TST+) (HIV+TST+), HIV negative TST+ (HIV−TST+), and HIV−TST− individuals.
Compared to HIV−TST−, latent TB infection led to increased levels of IP-10, IFN-γ and IL-17, while levels of IL-2 and IP-10 were increased with active TB. Levels of IFN-γ, IL-17, MIP-1α, and IL-10 were increased in HIV−TST+ individuals compared to HIV−TB+ patients. HIV coinfection decreased the level of IFN-γ, IL-17, IP-10 and IL-2. After six months (M6) of anti-TB treatment (ATT) in HIV−TB+ patients, IFN-γ, IL-10, and MIP-1α levels normalized. After M6 and M18 of ATT plus HAART in HIV+TB+ patients, levels of MIP-1α and IL-10 normalized, while this was not the case for IFN-γ, IL-2, IL-17, and IP-10 levels. In HIV+TST+ patients on HAART, levels of IFN-γ, IL-17, IL-10 and MIP-1α normalized, while no change in the levels of IL-2 and IP-10 were observed.
In conclusion, the simultaneous measurement of IFN-γ, IL-17 and IP-10 may assist in diagnosing LTBI; IL-2 and IP-10 may assist in diagnosing active TB; while IFN-γ, IL-17, MIP-1α, and IL-10 levels could help to discriminate LTBI and active TB. In addition, IL-10 and MIP-1α levels could help to monitor responses to TB treatment and HAART.
Le texte complet de cet article est disponible en PDF.Keywords : HIV/AIDS, Tuberculosis, Cytokines, Biomarkers
Plan
Vol 96
P. 131-140 - janvier 2016 Retour au numéro
Article précédent
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