Durability of direct immunofluorescence (DIF) slides stored at room temperature - 12/11/15
Abstract |
Background |
Prior studies suggested that direct immunofluorescence (DIF) slides can be stored at room temperature.
Objectives |
We sought to determine the durability of DIF slides stored at room temperature for 5 years.
Methods |
This was a retrospective study of 83 DIF slides archived at room temperature during 2010. The pattern of immunoreactants was compared with those noted in the original report.
Results |
Loss of reactivity was limited to cases with weak fluorescence at original diagnosis. Loss of IgG was noted in 12.5% of cases, IgA in 12%, C3 in 10%, and IgM in 9.75%. Fibrin showed no loss of reactivity. Preservation of immunofluorescence was not related to site of deposition. Overall, a reliable diagnosis could be made in 75 of 79 archived cases (94.9%).
Limitations |
Cases had been archived for periods varying from 4.5 to 5 years. Variations in processing and fluorochromes could affect durability. We have no way of knowing how long slides had been exposed to ultraviolet light at the time of initial examination.
Conclusion |
DIF showed excellent durability in slides kept at room temperature for 5 years.
Le texte complet de cet article est disponible en PDF.Key words : direct immunofluorescence, room temperature, storing
Plan
| The first 2 authors contributed equally in this project. |
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| This work was partially supported by the China Scholarship Council, project No. 201406015016. |
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| Conflicts of interest: None declared. |
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| Reprints not available from the authors. |
Vol 73 - N° 6
P. 1021-1024 - décembre 2015 Retour au numéro
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