Long noncoding RNA expression patterns in lymph node metastasis in colorectal cancer by microarray - 12/10/15
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 7 |
| Iconographies | 5 |
| Vidéos | 0 |
| Autres | 0 |
Abstract |
Aim |
To profile the long noncoding RNA (lncRNA) expression patterns in normal lymph node (NLN) of colorectal cancer compared with paired metastatic lymph node (MLN).
Materials and methods |
MLN, NLN lncRNA and messenger RNA (mRNA) expression levels were determined by microarray in three patients; quantitative reverse transcription–polymerase chain reaction validation of 3 differentially expressed lncRNAs was conducted in another 26 patients. The lncRNAs’ functions were predicted through coexpressed mRNA annotations.
Result(s) |
A total of 1133 lncRNA transcripts transcripts were dysregulated in metastatic lymph node, compared with normal lymph node. The expressions of the 3 chosen lncRNAs were validated by quantitative reverse transcription–polymerase chain reaction. Functional analysis suggests that several groups of lncRNAs may participate in biological pathways related to colorectal cancer by cis- and/or trans-regulation of protein-coding genes.
Conclusion(s) |
This study constitutes the first report of lncRNA expression patterns in human MLN, NLN of colorectal cancer. More than 1000 dysregulated lncRNA transcripts are found by microarray.
Le texte complet de cet article est disponible en PDF.Keywords : Lymph node metastasis, Colorectal cancer, Long noncoding RNA, Microarray
Plan
Vol 75
P. 12-18 - octobre 2015 Retour au numéro
Article précédent
- The up-regulation of long non-coding RNA AFAP1-AS1 is associated with the poor prognosis of NSCLC patients
- Jun Deng, Yujia Liang, Chunfeng Liu, Shengdong He, Songping Wang
- Decreased miR-187 induces retinal ganglion cell apoptosis through upregulating SMAD7 in glaucoma
- Qiu-li Zhang, Wei Wang, Jin Li, Shi-Yuan Tian, Tian-Zi Zhang
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?