Chloride intracellular channel 1 is an important factor in the lymphatic metastasis of hepatocarcinoma - 24/04/12
Corresponding author. Tel.: +86 411 86110002; fax: +86 411 86118866.Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 6 |
| Iconographies | 4 |
| Vidéos | 0 |
| Autres | 0 |
Abstract |
We have previously demonstrated that chloride intracellular channel 1 (CLIC1) is involved in the lymphatic metastasis of tumors. In this study, a self-designed shRNA sequence of mouse CLIC1 gene was synthesized and inserted into a pGPU6/GFP/Neo plasmid, then stably transfected into mouse hepatic carcinoma cell line Hca-F cells to down-regulate the expression of CLIC1 gene. The levels of expression of CLIC1 mRNA and protein were detected by real-time quantitative polymerase chain reaction (qRT-PCR) and western blot (WB) analysis, respectively. The down-regulation of CLIC1 enhanced proliferative activity, increased the ratio of G2/M and decreased percentage of apoptosis. In addition, the capability of migration and invasion decreased significantly. The results indicate that CLIC1 is a critical factor in the development of lymphatic metastasis.
Le texte complet de cet article est disponible en PDF.Keywords : CLIC1, RNA interference, Lymphatic metastasis, Hepatocarcinoma
Plan
Vol 66 - N° 3
P. 167-172 - avril 2012 Retour au numéro
Article précédent
- Lobaplatin induces apoptosis and arrests cell cycle progression in human cholangiocarcinoma cell line RBE
- Zhaohai Wang, Xuelian Tang, Yilu Zhang, Ruizhao Qi, Zhiwei Li, Keming Zhang, Zhenwen Liu, Xinying Yang
- Therapy-related acute leukemia in two patients with multiple sclerosis treated with Mitoxantrone
- Natasa Colovic, Nada Suvajdzic, Nada Kraguljac Kurtovic, Vesna Djordjevic, Marija Dencic Fekete, Jelena Drulovic, Ana Vidovic, Dragica Tomin
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?