cagA status and virulence of Helicobacter pylori strains - 06/05/08
Gilles Bommelaer [1],
Stanislas Bruley Des Varannes [2],
Jean-François Fléjou [3],
Tamara Matysiak [4],
Thierry Poynard [5],
Alain Richard [6],
Alain Slama [7],
Francis Mégraud et le Groupe d'Etude HELIGASTRE [4],
[4]
Voir les affiliationsPrevious experimental and epidemiological studies with few patients suggested that the presence of the cagA gene was a virulence factor for Helicobacter pylori (H. pylori) .
Aim |
To establish in this large epidemiological cohort study the relationship between the histological virulence of H. pylori infection and the cagA status of the bacteria.
Methods |
This prospective cohort study (6 month follow-up) was conducted on adult patients undergoing endoscopy for upper gastrointestinal symptoms. The cagA status of H. pylori -positive patients was established using the polymerase chain reaction (PCR) method on an antral biopsy. A score of histological virulence (inflammation, activity) was recorded on the basis of the Sydney system (on antral, angular and fundic biopsies). Eradication treatment given was not imposed and a clinical follow-up was performed at 3 and 6 months. H. pylori eradication was verified by a 13 C urea breath test at 3 months.
Results |
Four hundred and twenty two centers recruited 652 patients (mean age: 51 ± 15 years, 55% female). Upper GI endoscopy was abnormal in 80% of the patients of whom 68% had a gastritis aspect; 38% were infected by H. pylori , and among them 51% were cagA -positive. The histological virulence scores associated with the cagA -positive strains were significantly higher than those associated with the cagA -negative strains, globally (P = 0.0035), in the antrum (P = 0.0063), and in the angulus (P = 0.046), but not in the fundus (P = 0.05). The cagA status was correlated neither with the symptom severity at inclusion and at 6 months (P > 0.05), nor with the H. pylori eradication rate at 3 months (75% in cagA -positive and 70% in cagA -negative strains, P = 0.52).
Conclusion |
This study on a large cohort of patients confirms the greater histological virulence of H. pylori cagA -positive strains. However, this virulence was not associated with more severe symptoms nor with an increase in resistance to H. pylori eradication treatment.
Keywords:
Helicobacter pylori
,
CagA
,
Virulence
Plan
© 2001 Elsevier Masson SAS. Tous droits réservés.
Vol 25 - N° 12
P. 1084-1089 - décembre 2001 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.