Molecular allergy diagnostics refine characterization of children sensitized to dog dander - 06/08/18
, Anna Asarnoj, MD, PhD b, c, Hans Grönlund, PhD d, Magnus P. Borres, MD, PhD e, f, Marianne van Hage, MD, PhD b, Gunnar Lilja, MD, PhD a, Jon R. Konradsen, MD, PhD b, cCet article a été publié dans un numéro de la revue, cliquez ici pour y accéder
Abstract |
Background |
Sensitization to dog dander is an important risk factor for rhinoconjunctivitis and asthma but is not sufficient for diagnosing dog allergy. Molecular allergy diagnostics offer new opportunities for refined characterization.
Objectives |
We sought to study the association between sensitization to all presently known dog allergen components and clinical symptoms of dog allergy in children evaluated by using nasal provocation tests (NPTs).
Methods |
Sixty children (age, 10-18 years) sensitized to dog dander extract underwent NPTs with dog dander extract. Measurement of IgE levels to dog dander and to Can f 1, Can f 2, Can f 3, and Can f 5 was performed with ImmunoCAP, and measurement of IgE levels to Can f 4 and Can f 6 was performed with streptavidin ImmunoCAP. An IgE level of 0.1 kUA/L or greater was considered positive.
Results |
There was an association between sensitization to an increasing number of dog allergen components and a positive nasal challenge result (P = .01). Sensitization to lipocalins (odds ratio [OR], 6.0; 95% CI, 1.04-34.5), in particular Can f 4 (OR, 6.80; 95% CI 1.84-25.2) and Can f 6 (OR, 5.69; 95% CI, 1.59-20.8), was associated with a positive NPT result. Monosensitization to Can f 5 was related to a negative NPT result (OR, 5.78; 95% CI, 1.01-33.0).
Conclusion |
Sensitization to an increasing number of dog allergen components and to lipocalins is associated with dog allergy. Monosensitization to Can f 5 should not be regarded primarily as a marker for dog allergy.
Le texte complet de cet article est disponible en PDF.Graphical abstract |
Key words : Allergy, Can f 1, Can f 2, Can f 3, Can f 4, Can f 5, Can f 6, children, dog, IgE, molecular allergology, nasal provocation test, sensitization
Abbreviations used : NPT, OR, ROC
Plan
| Supported by the Swedish Asthma and Allergy Association's Research Foundation, the Stockholm County Council (ALF project and clinical post-doc), the Swedish Research Council, the Swedish Heart-Lung Foundation, the Swedish Cancer and Allergy Foundation, the Hesselman foundation, the Konsul Th C Bergh foundation, and the Karolinska Institutet. |
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| Disclosure of potential conflict of interest: M. P. Borres is an employee of Thermo Fisher Scientific (Uppsala, Sweden). M. van Hage has received lecture fees from Thermo Fisher Scientific (Uppsala, Sweden) and consultancy fees from Biomay AG (Vienna, Austria) and Hycor Biomedical. J. R. Konradsen has received material from Thermo Fisher to perform the IgE analysis in this project. The rest of the authors declare that they have no relevant conflicts of interest. |
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